Accumulation of silver nanoparticles by cultured primary brain astrocytes
Identifieur interne : 001586 ( Main/Exploration ); précédent : 001585; suivant : 001587Accumulation of silver nanoparticles by cultured primary brain astrocytes
Auteurs : Eva M. Luther [Allemagne] ; Yvonne Koehler [Allemagne] ; Joerg Diendorf [Allemagne] ; Matthias Epple [Allemagne] ; Ralf Dringen [Allemagne, Australie]Source :
- Nanotechnology [ 0957-4484 ] ; 2011.
English descriptors
- Teeft :
- Agno3, Agnp, Ashing procedure, Astrocyte, Astrocyte cultures, Brain astrocytes, Brain cells, Bremen, Cell lysate, Cell lysates, Cell viability, Cells toxicol, Cellular, Cellular silver content, Chem, Culture medium, Cultured, Cultured astrocytes, Dringen, Endocytosis, Endocytosis inhibitors, Endocytotic, Endocytotic pathways, Glutathione, Gssg, Gure, Human mesenchymal, Incubation, Inhibitor, Iron oxide nanoparticles, Lung cancer cells, Lysate, Mesenchymal, Nanoparticle, Nanoparticles, Nanotechnology, Neurochem, Nmol, Nmol protein, Pathway, Propidium iodide, Protein content, Reactive oxygen species, Silver content, Silver nanoparticles, Substantial amounts, Toxicity, Toxicol, Viability.
Abstract
Silver nanoparticles (AgNP) are components of various food industry products and arefrequently used for medical equipment and materials. Although such particles enter thevertebrate brain, little is known on their biocompatibility for brain cells. To study theconsequences of an AgNP exposure of brain cells we have treated astrocyte-richprimary cultures with polyvinylpyrrolidone (PVP)-coated AgNP. The incubationof cultured astrocytes with micromolar concentrations of AgNP for up to 24hresulted in a time-and concentration-dependent accumulation of silver, but didnot compromise the cell viability nor lower the cellular glutathione content. Incontrast, the incubation of astrocytes for 4h with identical amounts of silver asAgNO3 already severely compromised the cell viability and completely deprived the cells ofglutathione. The accumulation of AgNP by astrocytes was proportional to theconcentration of AgNP applied and significantly lowered by about 30 in thepresence of the endocytosis inhibitors chloroquine or amiloride. Incubation at4C reducedthe accumulation of AgNP by 80 compared to the values obtained for cells that had been exposed toAgNP at 37C. These data demonstrate that viable cultured brain astrocytes efficiently accumulatePVP-coated AgNP in a temperature-dependent process that most likely involvesendocytotic pathways.
Url:
DOI: 10.1088/0957-4484/22/37/375101
Affiliations:
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amounts</term><term>Toxicity</term><term>Toxicol</term><term>Viability</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Silver nanoparticles (AgNP) are components of various food industry products and arefrequently used for medical equipment and materials. Although such particles enter thevertebrate brain, little is known on their biocompatibility for brain cells. To study theconsequences of an AgNP exposure of brain cells we have treated astrocyte-richprimary cultures with polyvinylpyrrolidone (PVP)-coated AgNP. The incubationof cultured astrocytes with micromolar concentrations of AgNP for up to 24hresulted in a time-and concentration-dependent accumulation of silver, but didnot compromise the cell viability nor lower the cellular glutathione content. Incontrast, the incubation of astrocytes for 4h with identical amounts of silver asAgNO3 already severely compromised the cell viability and completely deprived the cells ofglutathione. The accumulation of AgNP by astrocytes was proportional to theconcentration of AgNP applied and significantly lowered by about 30 in thepresence of the endocytosis inhibitors chloroquine or amiloride. Incubation at4C reducedthe accumulation of AgNP by 80 compared to the values obtained for cells that had been exposed toAgNP at 37C. These data demonstrate that viable cultured brain astrocytes efficiently accumulatePVP-coated AgNP in a temperature-dependent process that most likely involvesendocytotic pathways.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li></country><region><li>Brême (Land)</li></region><settlement><li>Brême</li></settlement></list><tree><country name="Allemagne"><region name="Brême (Land)"><name sortKey="Luther, Eva M" sort="Luther, Eva M" uniqKey="Luther E" first="Eva M" last="Luther">Eva M. Luther</name></region><name sortKey="Diendorf, Joerg" sort="Diendorf, Joerg" uniqKey="Diendorf J" first="Joerg" last="Diendorf">Joerg Diendorf</name><name sortKey="Dringen, Ralf" sort="Dringen, Ralf" uniqKey="Dringen R" first="Ralf" last="Dringen">Ralf Dringen</name><name sortKey="Dringen, Ralf" sort="Dringen, Ralf" uniqKey="Dringen R" first="Ralf" last="Dringen">Ralf Dringen</name><name sortKey="Dringen, Ralf" sort="Dringen, Ralf" uniqKey="Dringen R" first="Ralf" last="Dringen">Ralf Dringen</name><name sortKey="Dringen, Ralf" sort="Dringen, Ralf" uniqKey="Dringen R" first="Ralf" last="Dringen">Ralf Dringen</name><name sortKey="Epple, Matthias" sort="Epple, Matthias" uniqKey="Epple M" first="Matthias" last="Epple">Matthias Epple</name><name sortKey="Koehler, Yvonne" sort="Koehler, Yvonne" uniqKey="Koehler Y" first="Yvonne" last="Koehler">Yvonne Koehler</name><name sortKey="Koehler, Yvonne" sort="Koehler, Yvonne" uniqKey="Koehler Y" first="Yvonne" last="Koehler">Yvonne Koehler</name><name sortKey="Luther, Eva M" sort="Luther, Eva M" uniqKey="Luther E" first="Eva M" last="Luther">Eva M. Luther</name></country><country name="Australie"><noRegion><name sortKey="Dringen, Ralf" sort="Dringen, Ralf" uniqKey="Dringen R" first="Ralf" last="Dringen">Ralf Dringen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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